Microbiology: Laboratory Theory & Application, Essentials, 2e

By Lourdes Norman-McKay, Michael J. Leboffe, Burton E. Pierce  •  
 2022  
•  528
 Pages
Loose Leaf
Format • 
Print ISBN  9781640434004
• eBook ISBN  9781640434011
Suggested Student Retail Price

$48.40

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This newest addition to the best-selling Microbiology: Laboratory Theory & Application series of manuals provides an excellent value for courses where lab time is at a premium.  The Essentials edition is intended for courses populated by nonmajors and allied health students and includes exercises selected to reflect core microbiology laboratory concepts.

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Features
What's New in this Edition
Table of Contents
About the Authors
Instructor Resources
Features
  • Contains 48 exercises with a focus on fundamental lab skills, microbial growth, microscopy, staining, selective media, differential tests, and medical microbiology.
  • Theory and Application provide students with the general principles and everyday purpose of the labs.
  • In This Exercise clearly explains what students can expect as they work through the Lab Procedures.
  • Biochemical reactions for the organism and indicator reactions of the test depict the reactions taking place in the lab.
  • MaterialsMedium Recipes, and Procedures give students step-by-step instructions for conducting the labs. Biosafety levels are also included so that students are aware of the safety precautions necessary for each lab.
  • Tables of results help students organize and interpret results.
  • Data sheets provide students room to record their data and answer critical thinking questions.
  • High-qualityfull-color photographsmicrographs, and illustrations ensure that students have color-accurate visual representations of what they will see in the lab.
  • Photographs and procedural diagrams outline the steps necessary to correctly complete the lab. Directional arrows help ensure students use proper technique.
  • Clearly written, informative captions help ensure students use proper technique in the lab.

Instructor Resources Available: Login to view Instructor Resources

  • An online Instructor's Manual contains tips for the instructor and answers to all of the exercises.
  • All of the images from the lab manual are provided electronically. Images are presented at digital display resolution (150 dpi), intended for use in PowerPoint presentations.
  • Digital Test Bank contains questions to help instructors build tests and quizzes that can either be printed or exported to commonly used distance learning platforms.
  • Available on the Top Hat active learning platform
What's New in this Edition
  • New Author! Dr. Lourdes Norman-McKay has been added to the author team. Dr. Norman-McKay is a professor at Florida State College at Jacksonville. Her experience as an educator, an author, an editor, a researcher, and an administrator gives her a unique perspective. We are delighted to add her voice to the market-leading content developed by Dr. Michael J. Leboffe and Burton E. Pierce.
  • The exercise order has been reorganized to more closely align with the order of a typical microbiology lecture/lab sequence.
  • Learning outcomes have been added.
  • Content has been subdivided into smaller sections for more flexibility and reduce cognitive load.
  • Lab Technique reference sheets have been added to allow students to easily refer to them when performing these techniques in later labs.
  • The Background and Clinical Application sections were modified to better meet students where they are and to explain to them what they need to know to be successful in a clinical setting.
  • Lab related questions have been diversified to include a more stratified representation of Bloom's learning taxonomy. In addition to the "in lab” documentation of findings, students now have access to multiple choice, true/false, labeling, short answer/fill in the blank, and critical thinking questions to support their review of content. These questions can be used as pre-lab and/or post-lab work.
  • Procedural diagrams now follow a linear format for easier comprehension.
  • Lab questions now include multiple choice, true/false, labeling, short answer/fill in the blank, and critical thinking questions.
  • Many references have been removed to save space. References have been added to footnotes only for material that is cutting edge and may not yet be widely known, for topics that are not general knowledge and that must really be investigated, and for hotly debated topics.
Table of contents
Safety in the Microbiology Laboratory
Section 1 Microscopy
Section 2 Aseptic Technique and Bacterial Growth and Enumeration
Section 3 Staining Bacteria
Section 4 Media Properties and Conditions that Affect Bacterial Growth
Section 5 Media for the Selection and/or Differentiation of Bacteria
Section 6 Biochemical Tests for Identify Bacterial Isolates
Section 7 Controlling Bacterial Growth
Section 8 Microbes Causing Trouble: Clinical Biofilms and Epidemics
Section 9 Identifying Unknowns
Appendix A: Biochemical Pathways
Appendix B: Miscellaneous Transfer Methods
Appendix C: Using a Glass Pipette
Appendix D: Using a Micropipette
Appendix E: Alternative Procedure for Exercise 10: Standard Plate Count
Appendix F: Morphological Features and Staining Results for Select Bacteria
Appendix G: Gram Stain and Cellular Morphology Flowchart
Appendix H: Identification Flowcharts for Gram-Positive Cocci and Rods
Appendix I: Identification Flowcharts for Enterobacteriaceae
Glossary
Index
About the Authors

Dr. Lourdes Norman-McKayis a professor at Florida State College Jacksonville, where peers and students recognized her with the Outstanding Faculty Award. After earning a degree in microbiology from the University of Florida, she completed a Ph.D. in biochemistry and molecular biology and a postdoctoral specialization in microbiology and immunology at the Pennsylvania State University College of Medicine. As a National Institutes of Health Postdoctoral Fellowship awardee, she studied the role of viruses in cancer. In her nearly two decades as a scientist-educator, she has trained thousands of healthcare professionals and secured extensive federal funding to promote STEM education and empower underrepresented groups in STEM. In addition to authoring globally recognized, market-leading texts in microbiology with Pearson and Morton Publishing (Top Hat), her considerable STEM program development experience ranges from designing and launching a biomedical sciences baccalaureate program to serving as a curriculum designer and subject matter expert for the Florida Space Research Institute and Workforce Florida. Dr. Norman-McKay also currently serves as a section editor for the American Society for Microbiology’s (ASM) Journal of Microbiology and Biology Education and is an active participant in ASM’s Microbiology in Nursing and Allied Health Committee.

As a speaker for the U.S. Department of State’s International Information Programs, Dr. Norman-McKay served in diplomacy outreach to empower women and youth in STEM, bolster STEM education, and build STEM capacity in Central Asia. As a 2022-23 Jefferson Science Fellow, Dr. Norman-McKay will serve as a Senior Science Policy Advisor to the State Department’s Office of the U.S. Global AIDS Coordinator and Health Diplomacy.

Instructor Resources
  • An online Instructor's Manual contains tips for the instructor and answers to all of the exercises.
  • All of the images from the lab manual are provided electronically. Images are presented at digital display resolution (150 dpi), intended for use in PowerPoint presentations.
  • Digital Test Bank contains questions to help instructors build tests and quizzes that can either be printed or exported to commonly used distance learning platforms.
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